Why the cut-off depth of fluorescent filter should be OD6
In recent years, with the development of life science technology, fluorescence probe technology
Fluorescent microscope, Polarization fluorescence detection technology, Multiphoton fluorescence detection technology
PCR gene amplification, fluorescence quantitative detection, etc
Biomedical detection technologies are constantly emerging,
For the gradient of the fluorescent filter,
The background depth puts forward higher technical requirements.
The duration of stimulated emission fluorescence of fluorescent reagent is very short
Emitting fluorescence brightness equivalent to excitation energy
One thousandth to one thousandth of a magnitude
Compared with the excited fluorescence, it is very weak
Both coexist in the test sample
And the excitation spectrum and emission spectrum wavelength gap is very small (about 15nm-30nm)
So we need to detect the light signal to excite the light in the fluorescence system
Deep isolation with emission light in the spectrum
Isolation degree reaches above OD4
To detect the fluorescent signal emitted by the sample
To improve the accuracy of fluorescence detection
Need to improve the isolation to above OD5
And the higher the isolation of the filter
The stronger the detection ability of micro fluorescence
The higher the detection accuracy of fluorescence microscope
So the core requirement of the filter of the fluorescence microscope is
High cut-off gradient, high transmittance of the filter, and cut-off of the background near the filter.
